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rbp sequences lacking a stop codon  (Addgene inc)


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    Structured Review

    Addgene inc rbp sequences lacking a stop codon
    Rbp Sequences Lacking A Stop Codon, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rbp+sequences+lacking+a+stop+codon/rbp+sequences+lacking+a+stop+codon/pmc07952577-293-4-12
    Average 90 stars, based on 1 article reviews
    rbp sequences lacking a stop codon - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Overcoming the design, build, test bottleneck for synthesis of nonrepetitive protein-RNA cassettes
    Article Snippet: After O/N growth, plasmids were extracted using a maxiprep kit (Agilent), their concentration was measured, and they were stored in a microcentrifuge tube in −20 °C. .. RBP sequences lacking a stop codon were amplified via PCR off either Addgene or custom-ordered templates (Genescript or IDT, see Supplementary Data ). .. MCP, PCP, and QCP were cloned into the RBP plasmid between restriction sites KpnI and AgeI, immediately upstream of a GFP gene lacking a start codon, under the pRhlR promoter (containing the rhlAB las box38) and induced by N-butanoyl-L-homoserine Lactone (C4-HSL, Cayman Chemical).

    Article Title: Overcoming the design, build, test (DBT) bottleneck for synthesis of nonrepetitive protein-RNA binding cassettes for RNA applications
    Article Snippet: After O/N growth, plasmids were extracted using a maxiprep kit (Agilent), their concentration was measured, and they were stored in an Eppendorf tube in −20. .. RBP sequences lacking a stop codon were amplified via PCR of either Addgene or custom-ordered templates (Genescript or IDT, see Extended Data Table 3). ..

    Article Title: Synthetic 5' UTRs Can Either Up- or Downregulate Expression upon RNA-Binding Protein Binding.
    Article Snippet: .. RBP sequences lacking a stop codon were amplified via PCR off either Addgene or custom-ordered templates (Genescript or IDT, see Table S2). ..

    Polymerase Chain Reaction:

    Article Title: Overcoming the design, build, test bottleneck for synthesis of nonrepetitive protein-RNA cassettes
    Article Snippet: After O/N growth, plasmids were extracted using a maxiprep kit (Agilent), their concentration was measured, and they were stored in a microcentrifuge tube in −20 °C. .. RBP sequences lacking a stop codon were amplified via PCR off either Addgene or custom-ordered templates (Genescript or IDT, see Supplementary Data ). .. MCP, PCP, and QCP were cloned into the RBP plasmid between restriction sites KpnI and AgeI, immediately upstream of a GFP gene lacking a start codon, under the pRhlR promoter (containing the rhlAB las box38) and induced by N-butanoyl-L-homoserine Lactone (C4-HSL, Cayman Chemical).

    Article Title: Overcoming the design, build, test (DBT) bottleneck for synthesis of nonrepetitive protein-RNA binding cassettes for RNA applications
    Article Snippet: After O/N growth, plasmids were extracted using a maxiprep kit (Agilent), their concentration was measured, and they were stored in an Eppendorf tube in −20. .. RBP sequences lacking a stop codon were amplified via PCR of either Addgene or custom-ordered templates (Genescript or IDT, see Extended Data Table 3). ..

    Article Title: Synthetic 5' UTRs Can Either Up- or Downregulate Expression upon RNA-Binding Protein Binding.
    Article Snippet: .. RBP sequences lacking a stop codon were amplified via PCR off either Addgene or custom-ordered templates (Genescript or IDT, see Table S2). ..



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